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Exploring new biomarkers in the tumour microenvironment of canine inflammatory mammary tumours
Author(s) -
Raposo T. P.,
Pires I.,
Prada J.,
Queiroga F. L.,
Argyle D. J.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12209
Subject(s) - carcinogenesis , cancer research , metastasis , inflammation , biology , real time polymerase chain reaction , inflammatory breast cancer , downregulation and upregulation , cancer , breast cancer , gene expression , pathology , gene , medicine , immunology , biochemistry
Human inflammatory breast cancer ( IBC ) and canine inflammatory mammary cancer ( CIMC ) are the most aggressive forms of mammary cancer. Current research aims to identify new therapeutic targets. Here, we investigated gene expression levels of biomarkers associated with the inflammatory microenvironment. A total of 32 formalin‐fixed paraffin‐embedded samples of canine mammary carcinoma ( CIMC = 26; non‐ CIMC = 6) were used and their cDNA subjected to quantitative polymerase chain reaction ( qPCR ) to establish gene expression levels for mediators commonly implicated in linking carcinogenesis with inflammation. Gene expression differences between CIMC and non‐ CIMC types were obtained for cyclooxygenase 2 ( COX ‐2) ( P = 0.004), synuclein gamma (SNCG) ( P = 0.006), tribbles 1 ( P = 0.025), vascular endothelial growth factor ( VEGF ) ( P = 0.017) and CSF1R ( P = 0.045). Among these biomarkers correlations were found, particularly between SNCG and tribbles 1 ( r = 0.512, P = 0.001). The efficient metastasis of CIMC is intimately linked to components in the tumour microenvironment. This study suggests that upregulation and correlation of SNCG and tribbles 1 deserves to be further explored.