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Circulating let‐7g is down‐regulated in Bernese Mountain dogs with disseminated histiocytic sarcoma and carcinomas – a prospective study
Author(s) -
Børresen B.,
Nielsen L. N.,
Jessen L. R.,
Kristensen A. T.,
Fredholm M.,
Cirera S.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12196
Subject(s) - medicine , biomarker , cancer , histiocytic sarcoma , microrna , pathology , urinalysis , prospective cohort study , oncology , histiocyte , biology , urine , biochemistry , gene
Cancer is a prevalent cause of mortality in Bernese mountain dogs ( BMDs ). Circulating microRNAs ( miRNAs ) are found in blood and have been identified as promising biomarkers in various neoplastic diseases in humans. In the current study, the expression profile of different types of miRNAs was investigated in healthy BMDs and BMDs with cancer. Seven healthy and six non‐treated BMDs with cancer [four with disseminated histiocytic sarcomas ( DHS )] were enrolled in this study. Clinical evaluations including physical examination, blood analysis, urinalysis and diagnostic imaging were performed on all dogs. Twenty‐four different miRNAs were profiled from RNA isolated from whole blood preserved in PAXgene ® tubes using quantitative real‐time PCR ( qPCR ). The miRNA let‐7g was significantly down‐regulated in dogs with cancer ( P = 0.002) and dogs with DHS ( P = 0.011) compared with healthy controls. This miRNA is a known tumour suppressor and further analyses are warranted to assess its value as a non‐invasive biomarker for early detection of different types of cancer in BMDs .