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Canine mammary carcinomas: influence of histological grade, vascular invasion, proliferation, microvessel density and VEGFR2 expression on lymph node status and survival time
Author(s) -
Diessler M. E.,
Castellano M. C.,
Portiansky E. L.,
Burns S.,
Idiart J. R.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12189
Subject(s) - proliferating cell nuclear antigen , pathology , lymph node , microvessel , lymph , medicine , vascular endothelial growth factor , kinase insert domain receptor , immunohistochemistry , biology , vascular endothelial growth factor a , vegf receptors
Spontaneous invasive non‐inflammatory canine mammary carcinomas ( CMC ) and their regional lymph nodes ( LN ) were analysed ( n = 136). Histological grade ( HG ) and vascular invasion ( VI ) in the tumours and lymph node status were recorded. Proliferation index ( PI ), microvessel density ( MVD ) and vascular endothelial growth factor receptor 2 ( VEGFR2 ) expression were estimated using anti‐proliferating cell nuclear antigen ( PCNA ), anti‐von Willebrand factor and anti‐Flk‐1, respectively. Eighteen months follow‐up was performed (34 bitches). Tumours of different grades showed differences regarding PI , Flk‐1/integrated optical density (Flk‐1/ IOD ) and MVD . Every feature showed significant association with LN status through bivariate analyses. From multivariate analyses, VI and Flk‐1/ IOD were selected to predict LN status. Data revealed that the probability of a CMC ‐bearing bitch to remain alive at 1, 4, 5 and 14–18 months was 0.91, 0.87, 0.81 and 0.77, respectively. Besides LN status, VI was the only feature positively correlated with survival time, although a trend to shorter survival of animal patients bearing high expressing VEGFR2 CMC was noted.