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Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high‐grade B‐cell lymphoma
Author(s) -
Poggi A.,
Miniscalco B.,
Morello E.,
Gattino F.,
Delaude A.,
Ferrero Poschetto L.,
Aresu L.,
Gelain M. E.,
Martini V.,
Comazzi S.,
Riondato F.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12184
Subject(s) - flow cytometry , lymphoma , medicine , canine lymphoma , pathology , cancer research , oncology , immunology
Ki67 can discriminate between high‐ and low‐grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67‐positive cells), evaluated by flow cytometry, in 40 dogs with high‐grade B‐cell lymphoma, treated with a modified Wisconsin–Madison protocol ( UW ‐25). The following variables were investigated for association with lymphoma specific survival ( LSS ) and relapse free interval ( RFI ): Ki67%, breed, sex, age, stage, substage, complete remission ( CR ). By multivariate analysis, Ki67% ( P = 0.009) and achievement of CR ( P = 0.001) were independent prognostic factors for LSS . Dogs with intermediate Ki67% (20.1–40%) presented longer LSS and RFI (median = 866 and 428 days, respectively) than dogs with low (median = 42 days, P < 0.001; median = 159 days, P = 0.014) or high (median = 173 days, P = 0.038; median = 100 days, P = 0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high‐grade B‐cell lymphoma.