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Osteosarcoma tissues and cell lines from patients with differing serum alkaline phosphatase concentrations display minimal differences in gene expression patterns
Author(s) -
Rodrigues L. C. de Sá,
Holmes K. E.,
Thompson V.,
Piskun C. M.,
Lana S. E.,
Newton M. A.,
Stein T. J.
Publication year - 2016
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12132
Subject(s) - osteosarcoma , alkaline phosphatase , biology , gene expression , cell culture , gene , microbiology and biotechnology , cancer research , enzyme , biochemistry , genetics
Abstract Serum alkaline phosphatase ( ALP ) concentration is a prognostic factor for osteosarcoma in multiple studies, although its biological significance remains incompletely understood. To determine whether gene expression patterns differed in osteosarcoma from patients with differing serum ALP concentrations, microarray analysis was performed on 18 primary osteosarcoma samples and six osteosarcoma cell lines from dogs with normal and increased serum ALP concentration. No differences in gene expression patterns were noted between tumours or cell lines with differing serum ALP concentration using a gene‐specific two‐sample t ‐test. Using a more sensitive empirical Bayes procedure, defective in cullin neddylation 1 domain containing 1 ( DCUN1D1 ) was increased in both the tissue and cell lines of the normal ALP group. Using quantitative PCR ( qPCR ), differences in DCUN1D1 expression between the two groups failed to reach significance. The homogeneity of gene expression patterns of osteosarcoma associated differing serum ALP concentrations are consistent with previous studies suggesting serum ALP concentration is not associated with intrinsic differences of osteosarcoma cells.