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Immunohistochemical expression of heat shock proteins, p63 and androgen receptor in benign prostatic hyperplasia and prostatic carcinoma in the dog
Author(s) -
Romanucci M.,
Frattone L.,
Ciccarelli A.,
Bongiovanni L.,
Malatesta D.,
Benazzi C.,
Brachelente C.,
Della Salda L.
Publication year - 2016
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12113
Subject(s) - immunostaining , heat shock protein , androgen receptor , immunohistochemistry , hyperplasia , pathology , basal (medicine) , basement membrane , hsp60 , chemistry , medicine , prostate cancer , cancer , hsp70 , gene , biochemistry , insulin
This study compared heat shock proteins Hsp60, Hsp72 and Hsp73, along with p63 and androgen receptor ( AR ) immunoexpression between 16 cases of benign prostatic hyperplasia ( BPH ) and 11 prostatic carcinomas ( PC a) in dogs. The proportion of Hsp60‐positive cells was higher in PC a compared with BPH ( P = 0.033), whereas the frequency and intensity of Hsp73 immunostaining did not differ significantly between the two groups. Hsp72‐immunostained nuclei formed a discontinuous layer along the basement membrane in BPH, whereas cells in this layer in PC a were negative or weakly positive. Hsp72 nuclear score showed significant positive associations with both p63 ( P = 0.016) and AR ( P = 0.009) scores. Double immunofluorescence revealed Hsp72‐p63 and Hsp72‐AR co‐expressions in basal cell nuclei. Aberrant cytoplasmic p63 immunolabelling was observed in 3 of 11 PC a cases. These results suggest a role of the combined expression of Hsp72, p63 and AR in basal epithelial cells in canine BPH and PC a.