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In vitro effects of Yunnan Baiyao on canine hemangiosarcoma cell lines
Author(s) -
Wirth K. A.,
Kow K.,
Salute M. E.,
Bacon N. J.,
Milner R. J.
Publication year - 2016
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12100
Subject(s) - tunel assay , apoptosis , in vitro , traditional medicine , hemangiosarcoma , pharmacology , terminal deoxynucleotidyl transferase , cell culture , medicine , chemistry , biology , pathology , biochemistry , angiosarcoma , genetics
Yunnan Baiyao is a Chinese herbal medicine that has been utilized for its anti‐inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated the in vitro activity of Yunnan Baiyao against three canine HSA cell lines after treatment with increasing concentrations of Yunnan Baiyao (50, 100, 200, 400, 600 and 800 µg mL −1 ) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC 50 ) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL −1 , respectively. Caspase‐3/7 activity increased in correlation with the IC 50 in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO‐BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found that Yunnan Baiyao causes dose and time dependent HSA cell death through initiation of caspase‐mediated apoptosis, which supports future studies involving Yunnan Baiyao .