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1,25‐Dihydroxyvitamin D 3 and its analogues increase catalase at the mRNA , protein and activity level in a canine transitional carcinoma cell line
Author(s) -
Middleton R. P.,
Nelson R.,
Li Q.,
Blanton A.,
Labuda J. A.,
Vitt J.,
Inpanbutr N.
Publication year - 2015
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12066
Subject(s) - calcitriol , catalase , superoxide dismutase , endocrinology , reactive oxygen species , medicine , chemistry , antioxidant , enzyme , microbiology and biotechnology , messenger rna , cell culture , calcitriol receptor , biology , biochemistry , vitamin d and neurology , gene , genetics
Antioxidant enzymes, such as catalase, superoxide dismutases ( SOD ), MnSOD and Cu/ ZnSOD , protect cells by scavenging reactive oxygen species ( ROS ). Numerous studies have reported the anti‐cancer effects of 1,25‐dihydroxyvitamin D 3 (calcitriol) and its related analogues, seocalcitol and analogue V. In this study, canine bladder transitional cell carcinoma ( cbTCC ) cells were used to determine effects of calcitriol and its related analogues on antioxidant enzyme gene expression, protein expression and activity. Catalase mRNA was increased in response to calcitriol (10 −7 M), and seocalcitol (10 −7 and 10 −9 M). MnSOD mRNA was decreased in response to calcitriol at 10 −7 M. Catalase was significantly increased in response to calcitriol (10 −7 and 10 −9 M), and seocalcitol (10 −9 M). Catalase enzymatic activity increased in response to calcitriol, seocalcitol and analogue V (10 −9 M). In addition, global gene expression analysis identified the involvement of mitogen‐activated protein kinase ( MAPK ) signalling in cbTCC 's response to calcitriol and seocalcitol treatment.