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Development and characterization of monoclonal antibodies to subgroup A avian leukosis virus
Author(s) -
Qiu Y.,
Li X.,
Fu L.,
Cui Z.,
Li W.,
Wu Z.,
Sun S.
Publication year - 2014
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12036
Subject(s) - monoclonal antibody , virology , retrovirus , virus , biology , avian leukosis , western blot , immunofluorescence , antibody , glycoprotein , microbiology and biotechnology , immunology , gene , biochemistry
Avian leukosis virus subgroup A ( ALV ‐A) is a retrovirus which infects egg‐type chickens and is the main pathogen of lymphoid leukosis ( LL ) and myeloid leukosis ( ML ). In order to greatly enhance the diagnosis and treatment of clinical avian leukemia, two monoclonal antibodies ( MAbs ) to ALV ‐A were developed by fusion between SP2 /0 and spleen cells from mice immunized with expressed ALV ‐A env‐gp85 protein. Using immunofluorescence assay ( IFA ), two MAbs reacted with ALV ‐A, but not with subgroups B and J of ALV . Western blot tests showed that molecular weight of ALV ‐A envelope glycoprotein recognized by MAbs was about 53 kD . Isotyping test revealed that two MAbs ( A5C1 and A4C8 ) were IgG1 isotypes. These MAbs can be used for diagnosis and epidemiology of ALV ‐A.