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Serial evaluation of neutrophil function in tumour‐bearing dogs undergoing chemotherapy
Author(s) -
LeBlanc A. K.,
LeBlanc C. J.,
Rohrbach B. W.,
Kania S. A.
Publication year - 2015
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12015
Subject(s) - respiratory burst , chemotherapy , neutrophile , medicine , sepsis , stimulation , lymphoma , oxidative phosphorylation , canine lymphoma , fissipedia , phorbol , gastroenterology , immunology , biology , inflammation , enzyme , biochemistry , protein kinase c
We hypothesized that neutrophil function in tumour‐bearing dogs is negatively impacted by chemotherapy. Flow cytometric techniques were used to assess neutrophil oxidative burst and phagocytic activities at baseline, 7 and 21 days after induction chemotherapy in 20 dogs with lymphoma. Dogs had a lower percentage of neutrophils exhibiting oxidative burst activity after stimulation with Escherichia coli (day 7; P = 0.009) and phorbol 12‐myristate 13‐acetate ( PMA ) (days 7 and 21; P = 0.0003 and P = 0.01, respectively), compared with healthy controls. From day 0 to 7, the percentage of neutrophils exhibiting oxidative burst activity decreased after stimulation with E. coli ( P = 0.016) and PMA ( P = 0.0006). Induction chemotherapy suppresses the percentage of neutrophils capable of oxidative burst in dogs with lymphoma, with improvement in phagocytic activity over time ( P = 0.03). The impact of neutrophil dysfunction on incidence and severity of sepsis in dogs receiving chemotherapy should be investigated.