Open AccessSubnormothermic machine perfusion for preservation of porcine kidneys in a donation after circulatory death model
Author(s) -
Hoyer Dieter P.,
Gallinat Anja,
Swoboda Sandra,
Wohlschläger Jeremias,
Rauen Ursula,
Paul Andreas,
Minor Thomas
Publication year - 2014
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/tri.12389
Subject(s) - machine perfusion , medicine , perfusion , creatinine , cold storage , transplantation , renal function , circulatory system , kidney , urology , donation , surgery , cardiology , liver transplantation , biology , horticulture , economics , economic growth
Summary Machine perfusion for preservation led to compelling success for the outcome of renal transplantation. Further refinements of methods to decrease preservation injury remain an issue of high interest. This study investigates functional and morphological aspects of kidneys preserved by subnormothermic (20 °C) machine perfusion ( SNTM ) compared with oxygenated hypothermic machine perfusion ( HMPox ) and cold storage ( CS ) in a donation after circulatory death ( DCD ) model. After 30 min of warm ischaemia, porcine kidneys were randomly assigned to preservation for 7 h by CS , HMPox or SNTM . Afterwards, kidneys were reperfused for 2 h with autologous blood in vitro for assessment of function and integrity. Application of SNTM for preservation led to significantly higher blood flow and urine output compared with both other groups. SNTM led to a twofold increased creatinine clearance compared with HMPox and 10‐fold increased creatinine clearance compared with CS . Structural integrity was best preserved by SNTM . In conclusion, this is the first study on SNTM for kidneys from DCD donors. SNTM seems to be a promising preservation method with the potential to improve functional parameters of kidneys during reperfusion.