Open AccessBlood dendritic cell levels associated with impaired IL ‐12 production and T ‐cell deficiency in patients with kidney disease: implications for post‐transplant viral infections
Author(s) -
Chen Ping,
Sun Qianmei,
Huang Yanfei,
Atta Mohamed G.,
Turban Sharon,
Segev Dorry L.,
Marr Kieren A.,
Naqvi Fizza F.,
Alachkar Nada,
Kraus Edward S.,
Womer Karl L.
Publication year - 2014
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/tri.12381
Subject(s) - medicine , elispot , immunology , t cell , transplantation , kidney transplantation , dendritic cell , cd8 , kidney disease , peripheral blood mononuclear cell , viremia , immune system , gastroenterology , virus , biology , in vitro , biochemistry
Summary Reduced pretransplant blood myeloid dendritic cell ( mDC ) levels are associated with post‐transplant BK viremia and cytomegalovirus ( CMV ) disease after kidney transplantation. To elucidate potential mechanisms by which m DC levels might influence these outcomes, we studied the association of m DC levels with m DC IL‐12 production and T‐cell level/function. Peripheral blood (PB) was studied in three groups: (i) end stage renal disease patients on hemodialysis ( HD ; n = 81); (ii) chronic kidney disease stage IV ‐ V patients presenting for kidney transplant evaluation or the day of transplantation ( E val/ T x; n = 323); and (iii) healthy controls ( HC ; n = 22). Along with a statistically significant reduction in m DC levels, reduced CD 8 + T ‐cell levels were also demonstrated in the kidney disease groups compared with HC . Reduced PB m DC and monocyte‐derived DC ( M o DC ) IL ‐12 production was observed after in vitro LPS stimulation in the HD versus HC groups. Finally, ELIS pot assays demonstrated less robust CD 3 + INF ‐γ responses by M o DC s pulsed with CMV pp65 peptide from HD patients compared with HC . PB m DC level deficiency in patients with kidney disease is associated with deficient IL ‐12 production and T ‐cell level/function, which may explain the known correlation of CD 8 + T ‐cell lymphopenia with deficient post‐transplant antiviral responses.