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Refrigeration of apheresis platelets in platelet additive solution ( PAS‐E ) supports in vitro platelet quality to maximize the shelf‐life
Author(s) -
Johnson Lacey,
Vekariya Shuchna,
Wood Ben,
Tan Shereen,
Roan Christopher,
Marks Denese C.
Publication year - 2021
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.16489
Subject(s) - apheresis , platelet , cold storage , shelf life , chemistry , blood product , platelet transfusion , plateletpheresis , andrology , food science , immunology , medicine , biology , surgery , horticulture
Abstract Background Refrigeration, or cold‐storage, of platelets may be beneficial to extend the limited shelf‐life of conventionally stored platelets and support transfusion protocols in rural and military areas. The aim of this study was to compare the morphologic, metabolic, and functional aspects of apheresis platelets stored at room‐temperature (RT) or cold conditions, in either plasma or supplemented with platelet additive solution (PAS). Study design and methods Double‐dose apheresis platelets were collected in either 100% plasma or 40% plasma/60% PAS‐E using the Trima apheresis platform. One component from each group was either stored at RT (20–24°C) or refrigerated (2–6°C). Platelets were tested over a 21‐day period. Results The platelet concentration decreased by approximately 30% in all groups during 21 days of storage ( p > .05). Cold‐storage reduced glycolytic metabolism, and the pH was maintained above the minimum specification (>6.4) for 21 days only when platelets were stored in PAS. The surface phenotype and the composition of the supernatant were differentially affected by temperature and storage solution. Functional responses (aggregation, agonist‐induced receptor activation, clotting time) were improved during cold‐storage, and the influence of residual plasma was assay dependent. Conclusion In vitro platelet quality is differentially affected by storage time, temperature, and solution. Cold‐storage, particularly in PAS, better maintains key metabolic, phenotypic, and functional parameters during prolonged storage.