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HLA molecule expression on the surface of cells and microparticles in platelet concentrates
Author(s) -
Pannetier Louise,
Tamagne Marie,
Bocquet Thibaut,
Pirenne France,
AnsartPirenne Hélène,
Vingert Benoît
Publication year - 2021
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.16201
Subject(s) - leukoreduction , human leukocyte antigen , platelet , flow cytometry , chemistry , cd63 , immunology , microbiology and biotechnology , antigen , biology , microvesicles , biochemistry , gene , microrna
Background Platelet (PLT) transfusions are an essential treatment for bleeding disorders. However, immunologic complications can occur, including alloantibody production against Class I HLA molecules. The principal source of HLA molecules in PLT concentrates (PCs) is the PLTs themselves. However, extracellular microparticles (MPs) present in PCs may express HLA molecules. Study Design and Methods We used nanoscale flow cytometry to explore the expression of HLA‐A2, HLA‐B7, and HLA‐B57 on the surface of cells, PLT‐derived MPs (PMPs), lymphocyte‐derived MPs (LMPs), and monocyte‐derived MPs (MMPs) present in PCs. Expression was studied during 7 days of storage. Results Platelets were not the only source of HLA molecules in PCs. HLA molecules were present on PMPs, LMPs, and MMPs. The level of HLA Class I molecule expression varied between haplotypes and MPs of different origins and during storage. Conclusion Platelets or residual cells remaining after leukoreduction are not the only source of HLA Class I molecules in PCs, highlighting the contribution of MPs to alloimmunization mechanisms. These data may be relevant for the development of new transfusion guidelines.