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Evaluation of the procoagulant properties of a newly developed platelet modified lysate product
Author(s) -
Refaai Majed A.,
Conley Grace W.,
Hudson Chad A.,
Spinelli Sherry L.,
Phipps Richard P.,
Morrell Craig N.,
Blumberg Neil,
McRae Hannah L.
Publication year - 2020
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.15844
Subject(s) - platelet , in vivo , lysis , thrombin , chemistry , blood product , platelet transfusion , thrombin generation , kinetics , platelet lysate , thrombosis , immunology , medicine , biochemistry , surgery , biology , microbiology and biotechnology , physics , quantum mechanics
BACKGROUND Platelet transfusion is associated with logistical problems with the national storage guidelines of platelets. This results in decreased function in vivo as a result of the platelet storage lesion, and complications such as allergic or hemolytic reactions and thrombosis. We evaluated a new, freshly prepared platelet modified lysate (PML) product designed to be more procoagulant than fresh and stored platelets. METHODS Fresh platelets were concentrated, sonicated, and centrifuged to produce PML. Samples of both washed and unwashed PML were evaluated for particle size, concentration, and activity, and then tested for clot kinetics and thrombin generation. PML samples were also stored at various temperatures for durations up to 6 months and evaluated for clot kinetics and thrombin generation throughout. RESULTS PML showed significantly higher concentration of platelet microparticles, increased procoagulant properties, and increased thrombin generation as compared to fresh and stored platelets. In addition, PML maintained its clot kinetics over a 6‐month storage period with variable storage conditions. CONCLUSIONS The newly proposed PML product is more procoagulant, stable, and has additional potential applications than currently available platelet products. Further studies will be performed to assess its functions in vivo and to assess thrombotic potential.