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Genotypic study of Citrobacter koseri , an emergent platelet contaminant since 2012 in France
Author(s) -
Emery Audrey,
Marpaux Nadine,
Naegelen Christian,
Valot Benoît,
Morel Pascal,
Hocquet Didier
Publication year - 2020
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.15617
Subject(s) - genome , biology , genotype , typing , phylogenetic tree , multilocus sequence typing , clone (java method) , population , outbreak , gene , genetics , microbiology and biotechnology , virology , medicine , environmental health
BACKGROUND Transfusion‐transmitted bacterial infection is a rare occurrence but the most feared complication in transfusion practices. Between 2012 and 2017, five cases of platelet concentrates (PCs) contaminated with the bacterial pathogen Citrobacter koseri (PC‐ Ck ) have been reported in France, with two leading to the death of the recipients. We tested the possibilities of the emergence of a PC‐specific clone of C. koseri ( Ck ) and of specific bacterial genes associated with PC contamination. STUDY DESIGN AND METHODS The phylogenetic network, based on a homemade Ck core genome scheme, inferred from the genomes of 20 worldwide Ck isolates unrelated to PC contamination taken as controls (U‐ Ck ) and the genomes of the five PC‐ Ck , explored the clonal relationship between the genomes and evaluated the distribution of PC‐ Ck throughout the species. Along with this core genome multilocus sequence typing approach, a Ck pan genome has been used to seek genes specific to PC‐ Ck isolates. RESULTS Our genomic approach suggested that the population of C. koseri is nonclonal, although it also identified a cluster containing three PC‐ Ck and eight U‐ Ck . Indeed, the PC‐ Ck did not share any specific genes. CONCLUSION The elevated incidence of PCs contaminated by C. koseri in France between 2012 and 2017 was not due to the dissemination of a clone. The determinants of the recent outbreaks of PC contamination with C. koseri are still unknown.