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Pathogen inactivation with amotosalen plus UVA illumination minimally impacts microRNA expression in platelets during storage under standard blood banking conditions
Author(s) -
Arnason Niels Arni,
Johannson Freyr,
Landrö Ragna,
Hardarsson Björn,
Irsch Johannes,
Gudmundsson Sveinn,
Rolfsson Ottar,
Sigurjonsson Olafur E.
Publication year - 2019
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.15575
Subject(s) - platelet , whole blood , platelet transfusion , buffy coat , pathogen , microrna , andrology , chemistry , immunology , biology , biochemistry , medicine , gene
BACKGROUND To reduce the risk of transfusion transmission infection, nucleic acid targeted methods have been developed to inactivate pathogens in PCs. miRNAs have been shown to play an important role in platelet function, and changes in the abundance of specific miRNAs during storage have been observed, as have perturbation effects related to pathogen inactivation (PI) methods. The aim of this work was to investigate the effects of PI on selected miRNAs during storage. STUDY DESIGN AND METHODS Using a pool and split strategy, 3 identical buffy coat PC units were generated from a pool of 24 whole blood donors. Each unit received a different treatment: 1) Untreated platelet control in platelet additive solution (C‐PAS); 2) Amotosalen‐UVA‐treated platelets in PAS (PI‐PAS); and 3) untreated platelets in donor plasma (U‐PL). PCs were stored for 7 days under standard blood banking conditions. Standard platelet quality control (QC) parameters and 25 selected miRNAs were analyzed. RESULTS During the 7‐day storage period, differences were found in several QC parameters relating to PI treatment and storage in plasma, but overall the three treatments were comparable. Out of 25 miRNA tested changes in regulation of 5 miRNA in PI‐PAS and 3 miRNA U‐PL where detected compared to C‐PAS. A statistically significant difference was observed in down regulations miR‐96‐5p on Days 2 and 4, 61.9% and 61.8%, respectively, in the PI‐PAS treatment. CONCLUSION Amotosalen‐UVA treatment does not significantly alter the miRNA profile of platelet concentrates generated and stored using standard blood banking conditions.