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A standardized kodecyte method to quantify ABO antibodies in undiluted plasma of patients before ABO‐incompatible kidney transplantation
Author(s) -
Perry Holly,
Bovin Nicolai,
Henry Stephen
Publication year - 2019
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.15247
Subject(s) - abo blood group system , serial dilution , plasmapheresis , transplantation , antibody , agglutination (biology) , kidney transplantation , medicine , immunology , antigen , pathology , alternative medicine
BACKGROUND The ABO transplantation barrier can be breached if antibody is reduced to low levels. Current serologic methods involve testing natural RBCs against dilutions of plasma to determine antibody levels, but these methods are poorly standardized and inherently error prone with consequent large inter‐ and intra laboratory variation. We evaluated the feasibility of using antigen‐standardized kodecytes and undiluted plasma as an alternative method for antibody measurement in patients preparing for ABO‐incompatible kidney transplantation. STUDY DESIGN AND METHODS A panel of five kodecytes, bearing defined levels of synthetic blood group A type 2 antigen was developed (kodecyte assay) to show reaction patterns against undiluted plasma that were indicative of anti‐A and anti‐A,B levels. This panel was evaluated against the contemporary method of testing dilutions of plasma against A 1 cells to determine titer (A 1 cell assay) in both column agglutination and tube techniques. Evaluation samples included reference standards, 102 group O plus 23 group B donors, and 40 pre‐ and post‐plasmapheresis samples from five prospective ABO‐incompatible kidney transplant patients. RESULTS Comparisons between the kodecyte and A 1 cell assays found greater than 90% correlation for all samples. Tube and column agglutination technology platform differences were observed with A 1 cells and kodecytes. Discordant samples were generally found to have high ratios of IgG:IgM or vice versa. CONCLUSIONS The kodecyte assay is a simple method that requires no sample dilution, and an optimized two‐cell kodecyte panel is potentially capable of informing ABO‐incompatible kidney transplantation decisions based on antibody levels.