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Agglutination testing for human erythrocyte product in the rhesus macaque
Author(s) -
Jackson Isabel L.,
Vicente Elisabeth,
Yazer Mark H.,
Spinella Philip C.
Publication year - 2019
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.15182
Subject(s) - rhesus macaque , nonhuman primate , human plasma , immunology , human blood , biology , macaque , agglutination (biology) , whole blood , virology , medicine , antibody , physiology , chemistry , paleontology , chromatography , evolutionary biology
There has been interest in using human blood products in nonhuman primate models of trauma to supplement human studies and to provide evidence to guide novel trauma resuscitation strategies. The compatibility of human RBCs has not been extensively studied in nonhuman primate species. METHODS Whole blood samples were collected from five healthy, nontransfused, not previously pregnant Chinese‐bred rhesus macaques. The whole blood was centrifuged, and the plasma was decanted from each sample. Group O‐negative human RBCs were mixed with the plasma from the rhesus macaque monkeys. Compatibility testing was performed by an immediate spin test and polyspecific and monospecific anti‐human globulin (AHG) tests in glass tubes. RESULTS Immediate spin testing revealed three out of five plasma samples (60%) from rhesus macaques caused at least 1+ agglutination with the human RBCs. Polyspecific anti‐human globulin (AHG) tests demonstrated that two of five plasma samples (40%) from rhesus macaques caused at least 1+ agglutination with the human RBC, while the monospecific AHG testing revealed that the incompatibility was caused by C3d, not IgG. CONCLUSION Human RBCs are not compatible with the plasma of some, but not all, Chinese‐bred rhesus macaques.