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Platelet storage in more than 90% additive solution containing glucose and bicarbonate has the potential to increase shelf life
Author(s) -
Bashir Saber,
Kemsley Kelly,
Min Kyungyoon,
Swann Ian D.,
Cardigan Rebecca
Publication year - 2018
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.14962
Subject(s) - platelet , buffy coat , chemistry , bicarbonate , shelf life , platelet activation , biochemistry , food science , biology , immunology , organic chemistry
BACKGROUND Platelet concentrates (PCs) suspended in more than 90% additive solution (AS) are recommended for patients with reactions to platelets stored in plasma. Next‐generation AS containing glucose and bicarbonate might enable storage of these PCs for longer than those in current‐generation AS, which was therefore evaluated in this study. STUDY DESIGN AND METHODS Five buffy coat or apheresis‐derived PCs were pooled and split into identical units. All units except the control were centrifuged, the plasma removed and replaced with AS (SSP+, PAS‐5, or PAS‐G), or resuspended in the same plasma and sampled 96 hours after resuspension for analysis. RESULTS Plasma carryover was less than 10%, total protein less than 1 g/unit, and immunoglobulin A levels lower than 0.1 mg/mL for all PCs in AS. The pH of all the platelets during storage was higher than 6.4. PAS containing glucose maintained superior in vitro platelet function during storage compared with those resuspended in SSP+. Compared with storage in SSP+, storage in PAS‐5 or PAS‐G resulted in better preservation of platelet adenosine triphosphate and hypotonic shock response, lower annexin V binding, and improved mitochondrial membrane potential. CONCLUSION Platelets resuspended in PAS‐5 and PAS‐G maintained in vitro function and metabolism during storage compared with SSP+ platelets. Elevated platelet metabolic activity was noticed in PAS‐G, and higher platelet activation was detected with PAS‐5. Platelets resuspended in PAS containing glucose has the potential to increase the shelf life of PC in more than 90% AS.