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Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies
Author(s) -
Kikuchi Go,
Kurita Ryo,
Ogasawara Kenichi,
Isa Kazumi,
Tsuneyama Hatsue,
Nakamura Yukio,
Yabe Ryuichi,
Shiba Masayuki,
Tadokoro Kenji,
Nagai Tadashi,
Satake Masahiro
Publication year - 2018
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.14840
Subject(s) - progenitor cell , antigen , antibody , monoclonal antibody , red blood cell , flow cytometry , biology , serology , microbiology and biotechnology , immunology , cell culture , monoclonal , stem cell , genetics
BACKGROUND Antibody screening in pretransfusion tests is necessary to avoid critical complications of blood transfusion. Although red blood cells (RBCs) expressing relevant alloantigen(s) have been used for serologic antibody screening, little attention has been given to the use of cell lines, in which blood group antigen gene(s) are transduced, as reagent RBCs for antibody screening. STUDY DESIGN AND METHODS The use of an erythroid progenitor cell line for serologic tests was studied. The expression of blood group antigens of erythroid progenitor cells was analyzed by genotyping and flow cytometry. Serologic analysis including hemagglutination was performed using erythroid progenitor cells to evaluate their sensitivity for antibody detection. Overexpression of exogenous erythroid antigen by lentiviral transduction was carried out and investigated for antibody detection sensitivity. RESULTS Erythroid progenitor cells contained a substantial amount of hemoglobin and expressed sufficient levels of blood group antigens to detect corresponding monoclonal antibodies. Furthermore, the cell line could acquire an exogenous RBC antigen after lentiviral transduction and detected corresponding monoclonal and alloantibodies with equal sensitivity to antigen‐positive RBCs. CONCLUSION Application of erythroid progenitor cell lines for screening for unexpected antibodies could be helpful in solving issues such as reagent availability associated with the conventional RBC‐based assay. The genetic expandability of erythroid progenitor cell lines by gene modification techniques could lead to the development of more convenient reagent RBCs.