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Reduction of Zika virus infectivity in platelet concentrates after treatment with ultraviolet C light and in plasma after treatment with methylene blue and visible light
Author(s) -
Fryk Jesse J.,
Marks Denese C.,
HobsonPeters Jody,
Watterson Daniel,
Hall Roy A.,
Young Paul R.,
Reichenberg Stefan,
Tolksdorf Frank,
Sumian Chryslain,
Gravemann Ute,
Seltsam Axel,
Faddy Helen M.
Publication year - 2017
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.14256
Subject(s) - infectivity , methylene blue , platelet , virus , virology , ultraviolet light , chemistry , detection limit , biology , chromatography , immunology , biochemistry , photochemistry , photocatalysis , catalysis
BACKGROUND Zika virus (ZIKV) has emerged as a potential threat to transfusion safety worldwide. Pathogen inactivation is one approach to manage this risk. In this study, the efficacy of the THERAFLEX UV‐Platelets system and THERAFLEX MB‐Plasma system to inactivate ZIKV in platelet concentrates (PCs) and plasma was investigated. STUDY DESIGN AND METHODS PCs spiked with ZIKV were treated with the THERAFLEX UV‐Platelets system at 0.05, 0.10, 0.15, and 0.20 J/cm 2 UVC. Plasma spiked with ZIKV was treated with the THERAFLEX MB‐Plasma system at 20, 40, 60, and 120 J/cm 2 light at 630 nm with at least 0.8 µmol/L methylene blue (MB). Samples were taken before the first and after each illumination dose and tested for residual virus. For each system the level of viral reduction was determined. RESULTS Treatment of PCs with THERAFLEX UV‐Platelets system resulted in a mean of 5 log reduction in ZIKV infectivity at the standard UVC dose (0.20 J/cm 2 ), with dose dependency observed with increasing UVC dose. For plasma treated with MB and visible light, ZIKV infectivity was reduced by a mean of at least 5.68 log, with residual viral infectivity reaching the detection limit of the assay at 40 J/cm 2 (one‐third the standard dose). CONCLUSIONS Our study demonstrates that the THERAFLEX UV‐Platelets system and THERAFLEX MB‐Plasma system can reduce ZIKV infectivity in PCs and pooled plasma to the detection limit of the assays used. These findings suggest both systems have the capacity to be an effective option to manage potential ZIKV transfusion transmission risk.