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Properties of donated red blood cell components from patients with hereditary hemochromatosis
Author(s) -
Sut Caroline,
HamzehCognasse Hind,
Laradi Sandrine,
Bost Vincent,
Aubrège Christine,
Acquart Sophie,
Vignal Martine,
Boutahar Nadia,
Arthaud Charles Antoine,
Ange Eyraud Marie,
Pozzetto Bruno,
Tiberghien Pierre,
Garraud Olivier,
Cognasse Fabrice
Publication year - 2017
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13890
Subject(s) - hereditary hemochromatosis , hemochromatosis , medicine , red blood cell , immunology , gastroenterology
BACKGROUND Red blood cells (RBCs) contain large amounts of iron, and periodic therapeutic phlebotomy is thus the main treatment for hereditary hemochromatosis (HH). However, the donation of therapeutic phlebotomy products from asymptomatic patients for transfusion purposes remains controversial. In this study, we compared the quality of RBCs obtained from HH patients with those of non‐HH RBCs, within the allowed 42‐day storage period. STUDY DESIGN AND METHODS RBCs were obtained from HH patient donors and random regular blood donors by whole blood collection. RBCs were stored for up to 42 days, according to national regulations and standard blood bank conditions in France. The following variables were assessed: hematologic and biochemical results, RBC membrane and soluble inflammatory markers, and the proinflammatory potential of HH RBC supernatant toward endothelial cells in an in vitro model. RESULTS There were no major differences between the two groups in terms of biophysical, biochemical, or soluble immunomodulatory factors. However, we observed small but significant differences in changes in RBC membrane proteins during storage, including increased phosphatidylserine expression and decreased hemolysis in HH compared with normal RBCs. However, there were no differences in terms of bioactivity of soluble immunomodulatory factors in the RBC supernatant during storage between HH and control donors, as determined by their effects on endothelial cells in vitro. CONCLUSIONS These in vitro studies suggest that RBCs from HH patients appear, while exhibiting subtle differences, to be suitable for transfusion purposes according to currently accepted criteria.