Premium
In vitro comparison between gamma‐irradiated cryopreserved and Day 7 liquid‐stored buffy coat–derived platelet components
Author(s) -
Crimmins Daryl,
Flanagan Peter,
Charlewood Richard,
Ruggiero Katya
Publication year - 2016
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13763
Subject(s) - cryopreservation , buffy coat , andrology , platelet , chemistry , in vitro , abo blood group system , blood product , thromboelastometry , immunology , microbiology and biotechnology , biochemistry , biology , medicine , pathology , embryo
BACKGROUND Cryopreserved platelet (PLT) components stored at −80°C in 5% to 6% dimethyl sulfoxide (DMSO) demonstrate enhanced hemostatic activity. Alterations in PLT surface glycoprotein expression and release of procoagulant microparticles during the freeze/thaw cycle result in PLT activation. Nothing is known of the effect of gamma irradiation on the in vitro quality of reconstituted cryopreserved PLTs. STUDY DESIGN AND METHODS Gamma‐irradiated (25‐50 Gy) buffy coat–derived PLT components were either stored at room temperature for 7 days (the current expiry in New Zealand) or cryopreserved at −80°C using 5% to 6% DMSO. Cryopreserved PLTs were thawed at 37°C and reconstituted in ABO‐identical plasma or PAS‐E and compared to Day 7 gamma‐irradiated liquid‐stored PLTs. In vitro assays were performed to assess glycoprotein expression, PLT functionality and soluble cytokine release. RESULTS Cryopreserved PLTs after thawing and reconstitution in ABO‐matched plasma or PAS‐E displayed differing recoveries (82.7 and 75.9%, respectively). Key expression levels of glycoproteins GPIbα (CD42b) and GPIIb (CD41a) were reduced. Cryopreserved PLTs retained the ability to form an effective functional clot, while showing accelerated initiation of clot formation (R‐time) compared to Day 7 gamma‐irradiated liquid‐stored PLTs. CONCLUSION Gamma‐irradiated buffy coat–derived liquid‐stored and cryopreserved PLTs have distinctly differing phenotypes. Cryopreserved PLTs reconstituted in ABO plasma have enhanced clot strength driven by coagulation factors and fibrinogen levels not present in PAS‐E. Irradiated cryopreserved PLTs maintain a similar in vitro quality profile and hemostatic behavior to previously published, nonirradiated cryopreserved PLTs.