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Effect of texture of platelet bags on bacterial and platelet adhesion
Author(s) -
Hadjesfandiari Narges,
Schubert Peter,
Fallah Toosi Salma,
Chen Zhongming,
Culibrk Brankica,
RamirezArcos Sandra,
Devine Dana V.,
Brooks Donald E.
Publication year - 2016
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13756
Subject(s) - adhesion , biofilm , staphylococcus epidermidis , platelet , platelet adhesion , chemistry , bacteria , platelet adhesiveness , microbiology and biotechnology , biophysics , food science , staphylococcus aureus , platelet aggregation , biology , immunology , genetics , organic chemistry
BACKGROUND Missed detection of Staphylococcus epidermidis contamination in platelet (PLT) storage bags by the standard 24‐hour‐postcollection BacT/ALERT screening test has been documented. A slow growth rate and the strong tendency of this bacterium to adhere to surfaces can contribute to missed detection of the pathogen. STUDY DESIGN AND METHODS Topography of two different PLT storage bag surfaces, textured (rough) and smooth surfaces of Terumo 80440 bags (designated A15), was studied. Adhesion of biofilm‐positive and ‐negative S. epidermidis strains on these surfaces was evaluated under static conditions. Quality of stored PLTs in A15 bags under blood bank conditions was compared for two different bag orientations (rough vs. smooth surface down) on Days 2, 5, and 7 of storage. PLT adhesion on the surfaces was evaluated after 7 days of storage. RESULTS Bacterial adhesion and biofilm formation were significantly higher on the rough surfaces of A15 bags compared to the smooth surfaces. After 7 days of storage in A15 bags, PLTs showed similar metabolite levels, pH, and response capacity in the bags with different orientation and more PLT adhesion and aggregation was observed on rough surfaces. CONCLUSION Higher bacterial adhesion on rough surfaces can contribute to missed detection of bacterial strains that tend to adhere on surfaces. PLT adhesion and aggregation on rough surfaces can affect the quality and safety of PLTs by promoting more bacterial adhesion and biofilm formation on surfaces.