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Low transfusion transmission of hepatitis E among 25,637 single‐donation, nucleic acid‐tested blood donors
Author(s) -
Harritshøj Lene H.,
Holm Dorte K.,
Sækmose Susanne G.,
Jensen Bitten A.,
Hogema Boris M.,
Fischer Thea K.,
Midgley Sofie E.,
Krog Jesper S.,
Erikstrup Christian,
Ullum Henrik
Publication year - 2016
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13700
Subject(s) - hepatitis e virus , blood transfusion , genotype , hepatitis e , virology , medicine , nucleic acid test , antibody , population , transmission (telecommunications) , biology , immunology , gene , covid-19 , disease , genetics , environmental health , infectious disease (medical specialty) , electrical engineering , engineering
BACKGROUND Hepatitis E virus genotype‐3 (HEV‐gt‐3) causes autochthonous infections in western countries, with a primary reservoir in animals, especially pigs. HEV transfusion transmission has been reported, and HEV‐gt‐3 prevalence is high in some European countries. The prevalence of HEV RNA was investigated among Danish blood donors, and the prevalence of HEV transfusion‐transmitted infection (TTI) was investigated among recipients. STUDY DESIGN AND METHODS Samples from 25,637 consenting donors collected during 1 month in 2015 were screened retrospectively using an individual‐donation HEV RNA nucleic acid test with a 95% detection probability of 7.9 IU/mL. HEV‐positive samples were quantified by real‐time polymerase chain reaction and genotyped. Transmission was evaluated among recipients of HEV RNA‐positive blood components. Phylogenetic analyses compared HEV sequences from blood donors, symptomatic patients, and swine. RESULTS Eleven donations (0.04%) were confirmed as positive for HEV RNA (median HEV RNA level, 13 IU/mL). Two donations were successfully genotyped as HEV‐gt‐3. Only one donor had a travel history outside Europe. Nine of 11 donors were male, but the gender ratio was nonsignificant compared with the total donor population. Seven available recipients tested negative for HEV RNA and anti‐HEV immunoglobulin M in follow‐up samples. One recipient was HEV RNA‐negative but anti‐HEV immunoglobulin G‐positive. HEV TTI was considered unlikely, but a transfusion‐induced secondary immune response could not be excluded. Phylogenetic analysis showed relatively large sequence differences between HEV from donors, symptomatic patients, and swine. CONCLUSIONS Despite an HEV RNA prevalence of 0.04% in Danish blood donations, all HEV‐positive donations carried low viral loads, and no evidence of TTI was found.