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Engraftment for CD34 selected stem cell products is not compromised by cryopreservation
Author(s) -
ReichSlotky Ronit,
Bachegowda Lohith S.,
Ancharski Michael,
Gergis Usama,
van Besien Koen,
Cushing Melissa M.
Publication year - 2016
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13435
Subject(s) - cryopreservation , umbilical cord , medicine , cd34 , stem cell , haematopoiesis , andrology , progenitor cell , cord blood , blood product , transplantation , leukapheresis , immunology , surgery , biology , embryo , genetics , microbiology and biotechnology
BACKGROUND The coinfusion of haploidentical CD34+ selected peripheral blood stem cell products with umbilical cord blood (UCB) provides early neutrophil recovery, long‐term UCB engraftment, and a lower incidence of graft‐versus‐host disease; however, this complex transplant presents a scheduling challenge for both the cellular therapy laboratory and the clinical team. Cryopreservation of the haploidentical product can facilitate scheduling, but has been previously shown to be associated with infusion reactions and delayed platelet (PLT) engraftment in allogeneic hematopoietic progenitor cell transplant. STUDY DESIGN AND METHODS To test whether cryopreservation of the CD34+ selected product compromises the graft, we compared neutrophil and PLT engraftment kinetics for patients receiving freshly infused or cryopreserved products. Seventy‐two products collected from haploidentical related donors were CD34+ selected and infused in a combined transplant with UCB: 32 were cryopreserved before infusion and 40 were infused fresh. RESULTS No adverse infusion events were reported in either group and there was no difference in neutrophil and PLT engraftment time between fresh and cryopreserved products. CONCLUSION Cryopreservation of a CD34+‐selected product can be safely used in a combined transplant with UCB and does not affect engraftment time.