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The impact of donor characteristics on the immune cell composition of mixture allografts of granulocyte–colony‐stimulating factor–mobilized marrow harvests and peripheral blood harvests
Author(s) -
Wang YuTong,
Zhao XiangYu,
Zhao XiaoSu,
Xu LanPing,
Zhang XiaoHui,
Wang Yu,
Liu KaiYan,
Chang YingJun,
Huang XiaoJun
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13251
Subject(s) - cd8 , cd3 , monocyte , immune system , granulocyte , immunology , t cell , medicine , bone marrow , lymphocyte , granulocyte colony stimulating factor , cytotoxic t cell , biology , in vitro , biochemistry , chemotherapy
BACKGROUND The association of donor characteristics with immune cell composition in allografts remains poorly understood. In this retrospective study, the effects of donor characteristics on immune cell composition in allografts were investigated. STUDY DESIGN AND METHODS The correlations of donor characteristics with the immune cell composition in mixture allografts of granulocyte–colony‐stimulating factor–mobilized marrow harvests and peripheral blood harvests of 390 healthy donors (male, 240; female, 150; median age, 40 years old) were analyzed. RESULTS The median doses of CD3+ T cells, CD4+ T cells, CD8+ T cells, CD3+CD4–CD8– T cells, and monocytes in mixture allografts were 160.57 × 10 6 , 89.29 × 10 6 , 56.16 × 10 6 , 10.87 × 10 6 , and 137.94 × 10 6 /kg, respectively. Multivariate analysis showed that younger donor age was associated with a higher dose of CD3+ T cells (p = 0.006), CD3+CD8+ T cells (p < 0.001), CD3+CD4–CD8– T cells (p = 0.004), and monocytes (p = 0.014), as well as a higher ratio of CD3+CD4–CD8– T cells/CD3+ T cells (p < 0.001) in the mixture allografts. A negative association of donor weight with CD3+ T cells (p < 0.001), CD4+ T cells (p = 0.002), CD8+ T cells (p < 0.001), and CD3+CD4–CD8– T cells (p = 0.044) was observed. The count of peripheral blood lymphocyte pre–peripheral blood apheresis was correlated with the yield of CD3+ T cells (p < 0.001) and CD4+ T cells (p = 0.001). The peripheral blood monocyte count before marrow harvest predicted the monocyte dose (p = 0.002). CONCLUSION The results suggested that older and overweight donors should not be chosen. The monocyte and lymphocyte counts before harvest could predict the yield of immune cells in allografts.