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Uric acid variation among regular blood donors is indicative of red blood cell susceptibility to storage lesion markers: A new hypothesis tested
Author(s) -
Tzounakas Vassilis L.,
Georgatzakou Hara T.,
Kriebardis Anastasios G.,
Papageorgiou Effie G.,
Stamoulis Konstantinos E.,
FoudoulakiPaparizos Leontini E.,
Antonelou Marianna H.,
Papassideri Issidora S.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13211
Subject(s) - uric acid , hemolysis , oxidative stress , antioxidant , red blood cell , chemistry , lesion , in vivo , red cell , protein carbonylation , whole blood , biochemistry , immunology , food science , andrology , biology , lipid peroxidation , medicine , pathology , microbiology and biotechnology
BACKGROUND Oxidative stress orchestrates a significant part of the red blood cell (RBC) storage lesion. Considering the tremendous interdonor variability observed in the “storability,” namely, the capacity of RBCs to sustain the storage lesion, this study aimed at the elucidation of donor‐specific factors that affect the redox homeostasis during the storage of RBCs in standard systems. STUDY DESIGN AND METHODS The hematologic profile of regular blood donors (n = 78) was evaluated by biochemical analysis of 48 different variables, including in vivo hemolysis and plasma oxidant and antioxidant factors and statistical analysis of the results. The possible effect of the uric acid (UA) variable on RBC storability was investigated in leukoreduced CPD/SAGM RBC units (n = 8) collected from donors exhibiting high or low prestorage levels of UA, throughout the storage period. RESULTS Among the hematologic variables examined in vivo, cluster analysis grouped the donors according to their serum UA levels. Plasma antioxidant capacity, iron indexes, and protein carbonylation represented covariants of UA factor. RBCs prepared by low‐ or high‐UA donors exhibited significant differences between them in spheroechinocytosis, supernatant antioxidant activity, and other RBC storage lesion–associated variables. CONCLUSION UA exhibits a storability biomarker potential. Intrinsic variability in plasma UA levels might be related to the interdonor variability observed in the storage capacity of RBCs. A model for the antioxidant effect of UA during the RBC storage is currently proposed.