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Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice
Author(s) -
Glier Hana,
Simak Jan,
Panigaj Martin,
Gelderman Monique P.,
Vostal Jaroslav G.,
Holada Karel
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13190
Subject(s) - flow cytometry , haematopoiesis , biotinylation , red blood cell , cd34 , microbiology and biotechnology , blood cell , biology , pathogenesis , immunology , stem cell
BACKGROUND Cellular prion protein (PrP C ) is expressed on various cell types including red blood cells (RBCs). The PrP C plays a key role in the pathogenesis of prion diseases, but its physiologic function remains unclear. PrP C is expressed on CD34+ hematopoietic stem cells and its expression is regulated during blood cell differentiation including the erythroid line. STUDY DESIGN AND METHODS We investigated the role of PrP C in RBC survival in circulation by transfusing a mix of biotin‐labeled RBCs from wild‐type (WT) and PrP knockout (KO) mice to groups of recipient mice (WT and KO). The proportion of biotinylated RBCs in peripheral blood was estimated by flow cytometry. RESULTS KO RBCs displayed a markedly higher first‐day posttransfusion recovery but had a decreased survival in circulation when compared to WT RBCs. Similar results were obtained in all groups of transfused mice, irrespective of RBCs biotinylation level. In addition, we confirmed this finding in an analogous study using Tga20 mice overexpressing PrP C and KO mice of a different genetic background. CONCLUSION Our results demonstrate that PrP C expression affects RBC recovery and survival in circulation.