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Dual roles of autologous CD8+ T cells in hematopoietic progenitor cell mobilization and engraftment
Author(s) -
Russell Athena,
Malik Sunita,
Litzow Mark,
Gastineau Dennis,
Roy Vivek,
Zubair Abba C.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13073
Subject(s) - cd34 , progenitor cell , cd8 , haematopoiesis , homing (biology) , mobilization , immunology , t cell , stem cell , cytotoxic t cell , biology , cancer research , chemistry , microbiology and biotechnology , immune system , biochemistry , history , ecology , archaeology , in vitro
BACKGROUND Poor marrow cellularity alone cannot explain poor hematopoietic progenitor cell (HPC) mobilization. This study assessed the role of CD8+ T cells in HPC cell mobilization and engraftment. STUDY DESIGN AND METHODS Mobilization and engraftment were assessed in 192 autologous HPC donors. CD34+, CD4+, and CD8+ T‐cell contents in apheresis products were evaluated. Using a chemotaxis assay, we assessed the effect of purified autologous CD8+ T cells from low and high mobilizers on HPC migration from high to low stromal cell–derived factor (SDF‐1α) concentration gradients. We also assessed CD8+ T‐cell content association with days to neutrophil engraftment. RESULTS The median number of CD34+ cells/kg was 4.7 × 10 6 . Patients were categorized according to their total CD34+ cell collection quartile distribution into low, moderate, and high mobilizers. We found that HPC products from low mobilizers contained more CD8+ T cells than HPC products from moderate and high mobilizers. Chemotaxis assays showed depletion of CD8+ T cells enhances HPC mobilization independent of SDF‐1α concentration. Neutrophil engraftment analysis showed that the higher the CD8+ T‐cell content per unit CD34+ cell, the faster the rate of engraftment. CONCLUSION Our findings suggest CD8+ T cells inhibit HPC mobilization and facilitate homing and engraftment.