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Viremia levels in hepatitis C infection among Egyptian blood donors and implications for transmission risk with different screening scenarios
Author(s) -
El Ekiaby Magdy,
Moftah Faten,
Goubran Heidi,
van Drimmelen Harry,
LaPerche Syria,
Kleinman Steve,
Busch Michael,
Lelie Nico
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13061
Subject(s) - viremia , window period , virology , hepatitis c virus , transmission (telecommunications) , medicine , serology , nat , hepatitis c , flaviviridae , seroconversion , immunology , viral load , antibody , biology , virus , computer network , computer science , electrical engineering , engineering
BACKGROUND Knowledge about the viral load (VL) distributions in different stages of hepatitis C virus (HCV) infection is essential to compare the efficacy of serologic screening and nucleic acid testing (NAT) in preventing transfusion transmission risk. We studied HCV‐RNA levels in Egyptian blood donors in the preseroconversion window period (WP) and in later anti‐HCV–positive stages of infection. STUDY DESIGN AND METHODS Subsets of individual‐donation (ID)‐NAT and anti‐HCV–yield samples from a screening study among 119,756 donors were tested for VL by quantitative polymerase chain reaction (qPCR). Low viremia levels below the quantification limit of qPCR were determined by probit analysis using the proportion of reactive results on replicate NATs. Poisson distribution statistics were used to estimate transmission risk in different stages of HCV infection based on 50% minimum infectious doses (MID 50 ) of 3.2 (1‐10) and 316 (100‐1000) virions in the absence and presence of anti‐HCV, respectively. RESULTS Rates of total HCV infections and WP‐NAT–yield donations in two Egyptian blood centers varied between 2.6% to 4.5% and 1:3100 to 1:9500, respectively. VLs ranged from 82 to 3 × 10 7 copies/mL in WP and from fewer than 1600 to 1.6 × 10 6 copies/mL in anti‐HCV–positive carrier donations. Only two (1.1%) of 175 donors with probable resolved infection had detectable RNA on replicate testing (estimated VLs of 0.5 and 1.8 copies/mL). This translates to an estimated transmission risk of 0.028% if ID‐NAT‐nonreactive, anti‐HCV–positive donations would be used for RBC transfusions. CONCLUSION Almost 99% of anti‐HCV–reactive donations without detectable HCV‐RNA on initial ID‐NAT screening had eradicated the virus from the circulation, while 1% had extremely low VLs and are likely not infectious. The incremental safety offered by serologic testing of ID‐NAT–screened blood seems minimal.

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