z-logo
Premium
Metagenomic profiling of the viromes of plasma collected from blood donors with elevated serum alanine aminotransferase levels
Author(s) -
Furuta Rika A.,
Sakamoto Hirotaka,
Kuroishi Ayumu,
Yasiui Kazuta,
Matsukura Harumichi,
Hirayama Fumiya
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.13057
Subject(s) - human virome , polymerase chain reaction , serology , biology , alanine aminotransferase , virology , alanine transaminase , metagenomics , immunology , medicine , andrology , antibody , genetics , gene
BACKGROUND In Japanese Red Cross (JRC) blood centers, blood collected from donors with serum alanine aminotransferase (ALT) levels of more than 60 U/L are disqualified even if serologically negative for transfusion‐transmitted infections (TTIs). To assess potential risks of TTIs in plasma with elevated serum ALT levels in the current donor screening program of the JRC, we conducted a metagenomic analysis (MGA) of virome profiles in the plasma of blood donors with or without elevated serum ALT levels. STUDY DESIGN AND METHODS Based on serum ALT levels, donors were classified into three groups: “high,” more than 79 U/L; “middle,” 61 to 79 U/L; and “low,” less than 61 U/L. We individually analyzed 100 plasma samples from each group by MGA, employing shotgun sequencing. Viral sequences detected using MGA were partly confirmed using real‐time polymerase chain reaction (PCR). RESULTS Donors with high and middle ALT levels were significantly younger than those with low ALT levels, and more than 90% were males. Herpesviridae , Anelloviridae , Picornaviridae , and Flaviviridae sequences were identified in plasma samples, and their distribution and frequency were not significantly different among the three groups. CONCLUSION The serum ALT test may be unsuitable for monitoring for additional risks of TTIs in blood donors who were negative for typical TTIs using serologic and nucleic acid tests. Although MGA is less sensitive than PCR, it remains the best technology to detect known viruses in these donors.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here