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Optimal method for collection of umbilical cord blood: an E gyptian trial for a public cord blood bank
Author(s) -
Bassiouny M.R.,
ElChennawi F.,
Mansour A.K.,
Yahia S.,
Darwish A.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12978
Subject(s) - cord blood , umbilical cord , in utero , medicine , cord , cd34 , transplantation , andrology , blood volume , stem cell , obstetrics , fetus , surgery , immunology , pregnancy , biology , genetics
Background Umbilical cord blood ( UCB ) contains stem cells and can be used as an alternative to bone marrow transplantation. Engraftment is dependent on the total nucleated cell ( TNC ) and CD34 + cell counts of the cord blood units. This study was designed to evaluate the effect of the method of collection of the UCB on the yield of the cord blood units. Study Design and Methods Informed consent was obtained from 100 eligible mothers for donation of cord blood. Both in utero and ex utero methods were used for collection. The cord blood volume was measured. The TNC and the CD34 + cell counts were enumerated. Results We have found that in utero collection gave significantly larger volumes of cord blood and higher TNC counts than ex utero collection. There was no significant difference between both methods regarding the CD34 + cell counts. This study revealed a significant correlation between the volume of the collected cord blood and both TNC and CD34 + cell counts. Conclusion It is better to collect cord blood in utero before placental delivery to optimize the quality of the cord blood unit.