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Routine storage of red blood cell ( RBC ) units in additive solution‐3: a comprehensive investigation of the RBC metabolome
Author(s) -
D'Alessandro Angelo,
Nemkov Travis,
Kelher Marguerite,
West F. Bernadette,
Schwindt Rani K.,
Banerjee Anirban,
Moore Ernest E.,
Silliman Christopher C.,
Hansen Kirk C.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12975
Subject(s) - metabolome , red blood cell , leukoreduction , metabolomics , glutamine , metabolism , shelf life , chemistry , biochemistry , biology , blood transfusion , food science , immunology , amino acid , chromatography
Background In most countries, red blood cells ( RBCs ) can be stored up to 42 days before transfusion. However, observational studies have suggested that storage duration might be associated with increased morbidity and mortality. While clinical trials are under way, impaired metabolism has been documented in RBCs stored in several additive solutions ( ASs ). Here we hypothesize that, despite reported beneficial effects, storage in AS ‐3 results in metabolic impairment weeks before the end of the unit shelf life. Study Design and Methods Five leukofiltered AS ‐3 RBC units were sampled before, during, and after leukoreduction D ay 0 and then assayed on a weekly basis from storage D ay 1 through D ay 42. RBC extracts and supernatants were assayed using a ultra–high‐performance liquid chromatography separations coupled online with mass spectrometry detection metabolomics workflow. Results Blood bank storage significantly affects metabolic profiles of RBC extracts and supernatants by D ay 14. In addition to energy and redox metabolism impairment, intra‐ and extracellular accumulation of amino acids was observed proportionally to storage duration, suggesting a role for glutamine and serine metabolism in aging RBCs . Conclusion Metabolomics of stored RBCs could drive the introduction of alternative AS s to address some of the storage‐dependent metabolic lesions herein reported, thereby increasing the quality of transfused RBCs and minimizing potential links to patient morbidity.