Premium
The effect of riboflavin and ultraviolet light on the infectivity of arboviruses
Author(s) -
Faddy Helen M.,
Prow Natalie A.,
Fryk Jesse J.,
Hall Roy A.,
Keil Shawn D.,
Goodrich Raymond P.,
Marks Denese C.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12899
Subject(s) - infectivity , virus , virology , transmission (telecommunications) , encephalitis , medicine , pathogen , infectious disease (medical specialty) , ross river virus , biology , disease , immunology , alphavirus , electrical engineering , engineering
Background Arboviruses are an emerging threat to transfusion safety and rates of infection are likely to increase with the increased rainfall associated with climate change. Arboviral infections are common in A ustralia, where R oss R iver virus ( RRV ), B armah F orest virus ( BFV ), and M urray V alley encephalitis virus ( MVEV ), among others, have the potential to cause disease in humans. The use of pathogen reduction technology ( PRT ) may be an alternative approach for blood services to manage the risk of arboviral transfusion transmission. In this study, the effectiveness of the M irasol PRT ( T erumo BCT ) system at inactivating RRV , BFV , and MVEV in buffy coat ( BC )‐derived platelets ( PLTs ) was investigated. Study Design and Methods BC ‐derived PLT concentrates in additive solution ( SSP +) were spiked with RRV , BFV , or MVEV and then treated with the M irasol PRT system. The level of infectious virus was determined before and after treatment, and the reduction in viral infectivity was calculated. Results Treatment with PRT ( M irasol) reduced the amount of infectious virus of all three arboviruses. The greatest level of inactivation was observed for RRV (2.33 log; 99.25%), followed by BFV (1.97 log; 98.68%) and then MVEV (1.83 log; 98.42%). Conclusion Our study demonstrates that treatment of PLT concentrates with PRT ( M irasol) reduces the infectious levels of RRV , BFV , and MVEV . The relevance of the level of reduction required to prevent disease transmission by transfusion has not been fully defined and requires further investigation. In the face of a changing climate, with its associated threat to blood safety, PRT represents a proactive approach for maintaining blood safety.