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Whole blood treated with riboflavin and ultraviolet light: quality assessment of all blood components produced by the buffy coat method
Author(s) -
Schubert Peter,
Culibrk Brankica,
Karwal Simrath,
Serrano Katherine,
Levin Elena,
Bu Daniel,
Bhakta Varsha,
Sheffield William P.,
Goodrich Raymond P.,
Devine Dana V.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12895
Subject(s) - buffy coat , riboflavin , blood component , whole blood , platelet , in vitro , andrology , blood product , ultraviolet light , platelet poor plasma , food science , chemistry , platelet rich plasma , medicine , surgery , immunology , biochemistry , intensive care medicine , photochemistry
Background Pathogen inactivation ( PI ) technologies are currently licensed for use with platelet ( PLT ) and plasma components. Treatment of whole blood ( WB ) would be of benefit to the blood banking community by saving time and costs compared to individual component treatment. However, no paired, pool‐and‐split study directly assessing the impact of WB PI on the subsequently produced components has yet been reported. Study Design and Methods In a “pool‐and‐split” study, WB either was treated with riboflavin and ultraviolet ( UV ) light or was kept untreated as control. The buffy coat ( BC ) method produced plasma, PLT , and red blood cell ( RBC ) components. PLT units arising from the untreated WB study arm were treated with riboflavin and UV light on day of production and compared to PLT concentrates ( PCs ) produced from the treated WB units. A panel of common in vitro variables for the three types of components was used to monitor quality throughout their respective storage periods. Results PCs derived from the WB PI treatment were of significantly better quality than treated PLT components for most variables. RBCs produced from the WB treatment deteriorated earlier during storage than untreated units. Plasma components showed a 3% to 44% loss in activity for several clotting factors. Conclusion Treatment of WB with riboflavin and UV before production of components by the BC method shows a negative impact on all three blood components. PLT units produced from PI ‐treated WB exhibited less damage compared to PLT component treatment.