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AS ‐7 improved in vitro quality of red blood cells prepared from whole blood held overnight at room temperature
Author(s) -
Veale Margaret F.,
Healey Geraldine,
Sran Amrita,
Payne Katherine A.,
Zia Majid,
Sparrow Rosemary L.
Publication year - 2015
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12779
Subject(s) - erythrocyte fragility , red blood cell , whole blood , chemistry , andrology , mannitol , annexin , glycophorin , tonicity , in vitro , medicine , hemolysis , biochemistry , immunology , membrane
Background Extended room temperature ( RT ) hold of whole blood ( WB ) may affect the quality of red blood cell ( RBC ) components produced from these donations. The availability of better RBC additive solutions ( AS s) may help reduce the effects. A new AS , AS ‐7 ( SOLX , H aemonetics C orporation), was investigated for improved in vitro quality of RBC s prepared from WB held overnight at RT . Study Design and Methods Sixteen WB units were held for 21.4 hours ± 40 minutes at 22°C on cooling plates before processing. Each pair of ABO ‐matched WB units were pooled, divided into a WB filter pack containing saline‐adenine‐glucose‐mannitol (control) and a LEUKOSEP WB ‐filter pack containing SOLX , and processed according to manufacturer's instructions. RBC s were stored at 2 to 6° C and sampled weekly until expiry. Glycophorin A ( GPA +) and annexin V –binding microparticles ( MP s) were quantitated using flow cytometry. Osmotic fragility, intracellular p H (p Hi ), adenosine triphosphate ( ATP ), 2,3‐diphosphoglycerate (2,3‐ DPG ), and routine quality variables were measured. Adhesion of RBC s to human endothelial cells ( EC s) was evaluated by flow perfusion under low shear stress (0.5 dyne/cm 2 ), similar to low blood flow in microvessels. Results ATP and 2,3‐ DPG levels were improved for SOLX ‐ RBC s. SOLX ‐ RBC s maintained higher p Hi , increased resistance to hypotonic stress, and reduced numbers of GPA + MP s. No significant difference was observed between annexin V binding to MP s or adhesion of RBC s to EC s under shear stress. Conclusion SOLX ‐stored RBC s showed increased osmotic resistance, p Hi , and reduced GPA + MP s and together with higher ATP and 2,3‐ DPG levels demonstrated improved in vitro RBC quality measures during 42 days of storage.