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Novel swine model of transfusion‐related acute lung injury
Author(s) -
Okazaki Hitoshi,
Ishikawa Osamu,
Iijima Takehiko,
Kohira Takahiro,
Teranishi Mai,
Kawasaki Shin,
Saito Akira,
Mikami Yu,
Sugiura Asuka,
Hashimoto Shiho,
Shimada Eiko,
Uchikawa Makoto,
Matsuhashi Mika,
Tsuno Nelson H.,
Tanaka Minoru,
Kiyokawa Nobutaka,
Fujimoto Junichiro,
Nagase Takahide,
Tadokoro Kenji,
Takahashi Koki
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12766
Subject(s) - medicine , transfusion related acute lung injury , lung , antibody , lipopolysaccharide , monoclonal antibody , gastroenterology , pathology , immunology , pulmonary edema
Background Transfusion‐related acute lung injury ( TRALI ) is a life‐threatening complication of blood transfusion. Antibodies against human leukocyte antigens in donors' plasma are the major causes of TRALI . Several animal models of TRALI have been developed, and the mechanism underlying TRALI development has been extensively investigated using rodent models. Although sheep models of nonimmune TRALI have been developed, large‐animal models of antibody‐mediated TRALI are not yet available. Study Design and Methods To develop a swine model of TRALI , male C lawn strain miniature pigs were used. A monoclonal antibody ( MoAb ) against swine leukocyte antigens ( SLA s) C lass I (4 G 8, 0.3 or 1.0 mg/kg body weight [ BW ]) and a control antibody (1.0 mg/kg BW ) were injected into the peripheral vein after priming with or without 1 μg/kg BW lipopolysaccharide ( LPS ; n = 3 each). Lung injury was assessed using PaO 2 / FiO 2 ( P / F ) ratio and by chest X ‐ray imaging. Histopathologic analysis was also conducted. Results Lung injury could be induced by injecting 4 G 8 at an amount of 1.0 mg/kg BW , after LPS . The P / F ratio 90 minutes after the administration of 4 G 8 significantly decreased (p < 0.05). Bilateral infiltration was shown in chest X ‐ray imaging. Lung injury was confirmed by histopathologic analysis. Conclusion Lung injury in pigs was successfully induced by anti‐ SLA MoAb . Priming with LPS is a prerequisite for inducing lung injury and the amount of the antibody is a critical condition.