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The CXCR 4 and adhesion molecule expression of CD 34+ hematopoietic cells mobilized by “on‐demand” addition of plerixafor to granulocyte–colony‐stimulating factor
Author(s) -
Girbl Tamara,
Lunzer Verena,
Greil Richard,
Namberger Konrad,
Hartmann Tanja Nicole
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12632
Subject(s) - plerixafor , granulocyte colony stimulating factor , haematopoiesis , mobilization , cxcr4 , transplantation , progenitor cell , stem cell , medicine , cancer research , immunology , receptor , andrology , pharmacology , chemotherapy , biology , chemokine , microbiology and biotechnology , archaeology , history
Background Granulocyte–colony‐stimulating factor ( G ‐ CSF ) is routinely used for mobilization of hematopoietic stem and progenitor cells preceding autologous transplantation after high‐dose chemotherapy in hematologic malignancies. However, due to high mobilization failure rates, alternative mobilization strategies are required. Study Design and Methods Patients who poorly mobilized CD 34+ hematopoietic cells ( HC s) with G ‐ CSF additionally received the CXCR 4 antagonist plerixafor. The phenotype of CD 34+ HC s collected after this plerixafor‐induced “rescue” mobilization, in regard to adhesion molecule and CD 133, CD 34, and CD 38 expression in comparison to CD 34+ HC s collected after traditional G ‐ CSF administration in good mobilizers, was analyzed flow cytometrically. To confirm previous studies in our patient cohort, the efficiency of mobilization and subsequent engraftment after this “on‐demand” plerixafor mobilization were analyzed. Results Pronounced mobilization occurred after plerixafor administration in poor mobilizers, resulting in similar CD 34+ cell yields as obtained by G ‐ CSF in good mobilizers, whereby plerixafor increased the content of primitive CD 133+/ CD 34+/ CD 38– cells. The surface expression profiles of the marrow homing and retention receptors CXCR 4, VLA ‐4, LFA ‐1, and CD 44 on mobilized CD 34+ cells and hematopoietic recovery after transplantation were similar in patients receiving G ‐ CSF plus plerixafor or G ‐ CSF . Unexpectedly, the expression levels of respective adhesion receptors were not related to mobilization efficiency or engraftment. Conclusion The results show that CD 34+ HC s collected by plerixafor‐induced rescue mobilization are qualitatively equivalent to CD 34+ HC s collected after traditional G ‐ CSF mobilization in good mobilizers, in regard to their adhesive phenotype and engraftment potential. Thereby, plerixafor facilitates the treatment of poor mobilizers with autologous HC transplantation after high‐dose chemotherapy.