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Removal of biological response modifiers associated with platelet transfusion reactions by columns containing adsorption beads
Author(s) -
Tanaka Shigenori,
Hayashi Tomoya,
Tani Yoshihiko,
Hirayama Fumiya
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12542
Subject(s) - adsorption , lactate dehydrogenase , platelet , chemistry , biological response modifiers , chromatography , lysis , immunology , biochemistry , medicine , enzyme , immune system , organic chemistry
Background Biological response modifiers ( BRM s), such as soluble CD 40 ligand (s CD 40 L ); regulated upon activation, normal T ‐cell expressed, and secreted ( RANTES ); and transforming growth factor‐β1 ( TGF ‐β1), are released from platelets ( PLTs ) during storage and may trigger adverse effects after PLT transfusion. Although washing PLT s is effective at reducing the level of BRM s and the incidence of transfusion reactions, the washing procedure is time‐consuming and may induce PLT activation. Furthermore, some BRM s continue to accumulate during the storage of washed PLT s. A method to remove BRM s using adsorbent columns has not yet been developed. Study Design and Methods We evaluated the ability of columns packed with S elesorb and L iposorber beads, which are both clinically used, to remove BRM s from PLT concentrates ( PC s) stored for 5 days. The levels of these BRM s were determined before and after adsorption. Results The adsorption columns significantly reduced the levels of RANTES and s CD 40 L and partially reduced TGF ‐β1. There were no significant effects on PLT activation, aggregation, morphology, and plasma lactate dehydrogenase (an indicator of PLT lysis) levels, or hypotonic shock response. Adsorption, however, reduced the PLT recovery to approximately 60% of the untreated value. Conclusions This study showed that the levels of BRM s were substantially reduced using columns of clinically available adsorption beads. PLT functions and the quality of PC s were maintained after adsorption. The use of adsorption columns may be useful in reducing the incidence of nonhemolytic transfusion reactions.