Premium
Determination of thromboelastographic responsiveness in stored single‐donor platelet concentrates
Author(s) -
Bontekoe Ido J.,
Meer Pieter F.,
Korte Dirk
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12515
Subject(s) - thromboelastography , thrombelastography , platelet , whole blood , clot formation , diluent , chemistry , clot retraction , biomedical engineering , andrology , medicine , surgery , platelet aggregation , nuclear chemistry
Background Thromboelastography ( TEG ) is widely used in hospitals but less commonly in blood banks for evaluation of platelet ( PLT ) concentrates ( PC s). A TEG‐PC assay for testing fresh or stored PLT s must reflect the quality of the PLT s. The added value could be measurement of donor‐dependent PLT quality. Study Design and Methods Whole blood ( WB ) normal values were generated from 100 donors, using standard tests. Nineteen single‐donor PC s were evaluated with a TEG‐PC assay, using O ctaplas as microparticle‐free diluent and kaolin or collagen as activator, stored up to 12 days, and also sampled for additional in vitro tests. Results WB values showed larger reaction rates ( R ‐ and K ‐times, angle) compared to the reference values and almost similar maximum amplitude ( MA ). PCs showed usual storage lesion and TEG‐PC results showed significant decreasing R ‐ and K ‐times and increasing angle. Mean MA values remained constant but individual measurements were affected by clot retraction. TEG tracings of two PCs with good quality on D ay 12 showed weak to strong clot retraction, while two PCs with poor quality showed moderate clot retraction on D ay 1, no clot retraction on D ays 5 to 12, and a decreased MA on D ay 12. Clot strength ( MA ) and especially clot retraction represent possibly donor‐specific effects. Conclusion A TEG‐PC assay has been developed that is sensitive to storage effects. The assay has the potential to be helpful in selection of PLT donors but needs improvement to be more sensitive, reproducible, and distinctive to determine whose PLTs store poorly and whose store well.