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The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling
Author(s) -
Kurach Jayme D.R.,
Almizraq Ruqayyah,
Bicalho Beatriz,
Acker Jason P.,
Holovati Jelena L.
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12490
Subject(s) - phosphatidylserine , rejuvenation , hemolysis , cold storage , red blood cell , chemistry , andrology , mannitol , cryopreservation , hypoxanthine , sphingomyelin , biochemistry , biology , cholesterol , immunology , medicine , membrane , microbiology and biotechnology , phospholipid , surgery , enzyme , embryo , horticulture
Background Our previous studies showed that hypothermic storage ( HS ) induces red blood cell ( RBC ) microparticle ( RMP ) generation and changes in phosphatidylserine ( PS ) and CD 47 expression on RBC s and RMP s. The aim of this study was to evaluate the effect of cold rejuvenation treatment at multiple time points during storage on these prehemolytic indicators of RBC membrane storage lesion. Study Design and Methods Leukoreduced RBC units in saline‐adenine‐glucose‐mannitol were used to generate three groups: untreated controls, sham‐treated units, and units treated with a cold (1‐6° C ) rejuvenation solution on D ay 28, 35, or 42 of HS . Units were assessed for hemolysis, adenosine triphosphate ( ATP ) concentration, lipid composition, and RMP generation, as well as PS and CD 47 expression throughout 49 days of HS . Results Rejuvenation treatment led to a significant increase in ATP concentration in all units, irrespective of treatment day. There were no significant differences between sham‐ and rejuvenation‐treated RBC samples in the levels of PS externalization, CD 47 expression, or the rate of RMP formation. RBC s rejuvenated on D ay 28 were enriched in glycerophosphocholine (+23.5%), depleted in sphingomyelin (−14%), and slightly depleted in cholesterol (−3.5%). Conclusion Cold rejuvenation in hypothermically stored RBC s affects the lipid composition of RBC s and respective RMP s in a time‐dependent fashion.