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Quality of red blood cells washed using an automated cell processor with and without irradiation
Author(s) -
Hansen Adele L.,
Turner Tracey R.,
Yi QiLong,
Acker Jason P.
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12489
Subject(s) - hemolysis , irradiation , hematocrit , mannitol , chemistry , hemoglobin , red blood cell , saline , andrology , chromatography , medicine , biochemistry , immunology , anesthesia , physics , nuclear physics
Background Sterile washing of red blood cells ( RBC s) and use of an additive solution permits longer postwash storage. The effect of irradiation during this extended storage time is unclear. Study Design and Methods RBC s were washed 14 days after collection using an automated cell processor and stored in saline‐adenine‐glucose‐mannitol. To determine how long washed and irradiated RBC s could be stored, units were irradiated 1, 4, 5, and 7 days after washing and in vitro quality was assessed. Determined limits of postwash storage time for washed and washed and irradiated RBC s were validated. Quality assessment included percent recovery, hemoglobin ( Hb ), hemolysis, extracellular K + , and adenosine triphosphate. Immunoglobulin ( Ig ) A levels were measured in the nonirradiated arm. Results RBC s irradiated 1 and 4 days after washing had unacceptably high hemolysis by D ay 7 postwash, not meeting the acceptance criterion (<0.8% hemolysis in 98% of units with 95% confidence). Therefore, a 48‐hour maximum storage time after irradiation was chosen. Storage limits tested in the validation phase were as follows: washing on D ay 14 and subsequent storage for 7 days (washed RBC s) and washing on D ay 14, irradiation on D ay 19, and subsequent storage for 48 hours (washed and irradiated RBC s). All units met criteria for Hb , hematocrit, hemolysis, and sterility for washed RBC s. However, RBC s were washed with less than 2 L of saline, and IgA levels in 27 of 40 units were too high to be suitable for transfusion to IgA ‐deficient recipients. Conclusion The extended expiry for washed and washed and irradiated RBC s met requirements for all indications except transfusion to IgA ‐deficient recipients.