Premium
Total nucleated cells as a sole predictor of distinct targets of hematopoietic potential ( CD 34+ cells) in cord blood units: the results of a large series analysis in autologous cord blood units
Author(s) -
Mazzocchetti Daniele,
Berti Amy Maria,
Sartini Roberta,
Lucarini Alessandra,
Ragusa Giuseppe,
Caroli Matteo,
Pierelli Luca
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12458
Subject(s) - cd34 , cord blood , haematopoiesis , andrology , receiver operating characteristic , volume (thermodynamics) , cord , medicine , stem cell , immunology , biology , surgery , microbiology and biotechnology , physics , quantum mechanics
Background Rapid identification of eligible cord blood units ( CBUs ) for banking is an important issue in hematopoietic stem cell procurement. Distinct contents of CD 34+ cells in CBU can contribute to identify grafts that may be banked also for unrelated transplants or limited to family‐directed or autologous use. Study Design and Methods Considering thresholds of CD 34+ cell content of 3 × 10 6 , 2 × 10 6 , and 1 × 10 6 CD 34+ cells, we analyzed a consecutive series of 1309 CBUs . CBUs were collected for autologous banking without any volume‐based preselection criteria. Predictors of distinct content of CD 34+ cells have been assessed by receiver operating characteristic ( ROC ) curve analysis. Results Median total nucleated cell ( TNC ) and CD 34+ cell counts of the series were 6.97 × 10 8 (range, 0.36 × 10 8 ‐34.9 × 10 8 ) and 1.47 × 10 6 (0‐20.56 × 10 6 ). Volumes ranged from 21 to 163 mL , with a median of 73.8 mL . For the CD 34+ target of 1 × 10 6 , the best predictor was TNC count with a threshold of 6.63 × 10 8 ; volume results were less predictive with a value of 68.1 mL . For CD 34+ targets of 2 × 10 6 and 3 × 10 6 , ROC curves confirmed a stronger predictive power of TNC , above the collected volume, with thresholds of 7.55 × 10 8 and 8.98 × 10 8 . ROC analysis by combining all predictors ( TNC , volume, TNC 2 , volume 2 , age of mothers, types of delivery, birthweight) gave worse results than TNC count alone. Conclusions This analysis, carried out on a large, unrestricted CBU series, shows that TNC alone is the best predictor of distinct targets of hematopoietic potential with the chance to predict CBU potentially useful for unrelated recipients or limited for family‐directed or autologous use.