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Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype
Author(s) -
Masiello Francesca,
Tirelli Valentina,
Sanchez Massimo,
Akker Emile,
Gabriella Girelli,
Marconi Maurizio,
Villa Maria Antonietta,
Rebulla Paolo,
Hashmi Ghazala,
Whitsett Carolyn,
Migliaccio Anna Rita
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12391
Subject(s) - cord blood , progenitor cell , buffy coat , red blood cell , antigen , andrology , peripheral blood mononuclear cell , immunology , microbiology and biotechnology , blood transfusion , blood cell , biology , chemistry , stem cell , medicine , in vitro , biochemistry , genetics
Background Cultured red blood cells ( cRBC s) from cord blood ( CB ) have been proposed as transfusion products. Whether buffy coats discarded from blood donations (adult blood [ AB ]) may be used to generate cRBC s for transfusion has not been investigated. Study Design and Methods Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts ( ERYs ) and cRBC s generated in human erythroid massive amplification ( HEMA ) culture by CB (n = 7) and AB (n = 33, three females, three males, one AB with rare blood antigens cryopreserved using CB protocols) were compared. Results Variability was observed both in progenitor cell content (twofold) and number of ERYs generated (1 log) by CB and AB in HEMA . The average progenitor cell contents of the subset of AB and CB analyzed were similar. AB generated numbers of ERY s three times lower (p < 0.01) than CB in HEMA containing fetal bovine serum but similar to CB in HEMA containing human proteins. Female AB contained two times fewer (p < 0.05) erythroid progenitor cells but generated numbers of ERY s similar to those generated by male AB . Cryopreserved AB with a rare blood group phenotype and shipped to another laboratory generated great numbers of ERY s, 90% of which matured into cRBC s. Blood group antigen expression was consistent with the donor genotype for ERY s generated both by CB and AB but concordant with that of native RBC s only for cells derived from AB . Conclusion Buffy coats from regular donors, including a donor with rare phenotypes stored under conditions established for CB , are not inferior to CB for the generation of cRBC s.