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Immune‐reactive soluble OX 40 ligand, soluble CD 40 ligand, and interleukin‐27 are simultaneously oversecreted in platelet components associated with acute transfusion reactions
Author(s) -
HamzehCognasse Hind,
Damien Pauline,
Nguyen Kim Anh,
Arthaud CharlesAntoine,
Eyraud MarieAnge,
Chavarin Patricia,
Absi Léna,
Osselaer JeanClaude,
Pozzetto Bruno,
Cognasse Fabrice,
Garraud Olivier
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12378
Subject(s) - leukoreduction , proinflammatory cytokine , platelet , apheresis , immunology , immune system , inflammation , medicine , interleukin , chemistry , cytokine
Background Leukoreduction of labile blood components dramatically decreases the frequency of minor, intermediate, and severe adverse events ( AE s), referred to as acute transfusion reactions ( ATR s), especially after transfusion of platelet components ( PC s). The pathophysiology of AE s may result from accumulation of soluble, secreted, platelet (PLT) factors with proinflammatory functions stored in PC s. Thus, several cosynergizing factors associated with PLT accumulation in PC s may contribute to clinically reported ATR s with inflammatory symptoms. Study Design and Methods We screened for 65 PLT‐associated secretory products in PCs that caused ATR s and identified PLT molecules associated with ATRs and inflammation. A functional in vitro study using PC supernatants assayed on reporting immune cells was performed to indicate relevance. Results Among 10,600 apheresis PC s, 30 caused inflammatory ATR s and contained significantly elevated levels of soluble CD 40 ligand (sCD40 L ), interleukin ( IL )‐27, and soluble OX40 ligand (sOX40L). Normal PLT s secreted IL ‐27 and s OX 40L at bioactive concentrations upon thrombin stimulation and were up regulated in association with ATR s, similar to sCD 40 L . Other secreted products were identified but not investigated further as their positivity was not consistent. Conclusions This study demonstrates the putative participation of PLT ‐derived s OX 40 L , IL ‐27, and sCD 40 L , which accumulate in PC supernatants, with inflammatory‐type ATRs . Further studies are required to determine the clinical significance of these findings to forecast preventive measures whenever possible.