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Random aggregates in newly produced platelet units are associated with platelet activation and release of the immunomodulatory factors sCD 40 L and RANTES
Author(s) -
Sandgren Per,
Meinke Stephan,
Eckert Elias,
Douagi Iyadh,
Wikman Agneta,
Höglund Petter
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12345
Subject(s) - platelet , chemistry , platelet activation , andrology , platelet aggregation , immunology , microbiology and biotechnology , biology , medicine
Background In connection with platelet ( PLT ) production, random but transient aggregates sometimes form in the newly produced units. The underlying mechanisms as well as the impact on cellular level of this phenomenon are unknown. Hypothetically, random occurrence of aggregates may induce biochemical changes leading to PLT activation and release of immunomodulatory factors from the PLTs . Study Design and Methods PLTs were aliquoted and prepared with an automated system for PLT pooling ( O rbi S ac, Terumo BCT) for a three‐arm nonpaired study design (n = 8). Initially aggregated PLT units in SSP + were selected by visual inspection and compared to unaffected PLT units stored in SSP + or 100% plasma. Cellular, metabolic, and functional variables were analyzed, including the concentrations of RANTES , sCD 40 L , and sTWEAK in the bags during a 9‐day storage period. Results Isolated aggregated PLTs show signs of spontaneous activation and respond less efficiently to TRAP stimulation. RANTES , sCD 40 L , and sTWEAK accumulated in the various PLT units during storage but sCD 40 L and RANTES accumulated in the initially aggregated PLT units to higher concentrations than the reference units and PLTs stored in 100% plasma (p < 0.001). Over time, the levels of sTWEAK increased more in the plasma storage environment compared with PLT units stored in SSP + (p < 0.001). Conclusion Our data indicate that random occurrence of aggregates may lead to higher activation level and increased release of immunomodulatory factors from the PLTs .