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Evaluation of an experimental filter designed for improving the quality of red blood cells (RBCs) during storage by simultaneously removing white blood cells and immunomodulators and improving RBC viscoelasticity and B and 3 proteins
Author(s) -
SowemimoCoker S.O.
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12330
Subject(s) - leukoreduction , hemolysis , antibody , andrology , red blood cell , band 3 , chemistry , platelet , immunology , biology , medicine , biochemistry , membrane protein , membrane
Background Storage age of red blood cells (RBCs) has been reported to be associated with increased mortality and morbidity. During storage, RBCs undergo changes in biochemical and functional properties. Stored RBCs may also contain white blood cells (WBCs), activated platelets (PLTs), cytokines, immunoglobulin, and other bioactive proteins. Transfusion of these bioactive proteins and cells with RBCs has the potential to cause serious adverse effects. We evaluated the performances of an experimental filter ( EF ) designed to remove immunoglobulins, cytokines, and other bioactive proteins in RBC s. Study Design and Methods Sixteen sets, each containing 3 units of ABO ‐identical RBCs in AS ‐3 were obtained from a blood bank. Three units of RBCs were combined together and then split into three equal aliquots, A, B, and C. Unit A was unfiltered while U nits B and C were filtered with a leukoreduction filter and the EF , respectively. All the units were stored at 4° C in a blood bank refrigerator for 42 days. We measured RBC viscoelasticity, hemolysis, RBC adenosine triphosphate, Band 3 proteins, cytokines, PLTs, WBCs, and immunoglobulin before and after filtration and on D ays 21 and 42 of storage. Data were analyzed by repeated‐measures analysis of variance with Newman‐Keuls multiple comparison test. Results The EF significantly (p < 0.05) reduced the levels of immunoglobulin (control IgG , 2.184 ± 1.918 mg/ mL ; BPF 4, 2.216 ± 1.956 mg/ mL ; and EF , 0.363 ± 0.391 mg/ mL ), PLTs, cytokines, and improved viscoelastic properties when compared to either control or leukoreduced RBCs . Conclusion The EF achieved lower levels of WBCs, improved viscoelastic properties, and reduced levels of immunoglobulins and cytokines but significance will require clinical evaluation.