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Pathogen reduction treatment alters the immunomodulatory capacity of buffy coat–derived platelet concentrates
Author(s) -
Loh Yen S.,
Johnson Lacey,
Kwok Matthew,
Marks Denese C.
Publication year - 2014
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/trf.12320
Subject(s) - buffy coat , platelet , peripheral blood mononuclear cell , immune system , lipopolysaccharide , immunology , secretion , andrology , transfusion medicine , chemistry , in vitro , biology , medicine , blood transfusion , biochemistry
Background Storage of platelet concentrates ( PCs ) after Mirasol pathogen reduction technology ( PRT ) treatment changes platelet ( PLT ) surface marker expression and secretion of immunomodulatory factors. Given that PLTs are known to participate in immune function, PRT may alter the way PLTs interact with the immune cells of a recipient upon transfusion. As such, the aim of this study was to assess the effects of PRT treatment on the functional ability of PLTs to interact with peripheral blood mononuclear cells ( PBMNCs ). Study Design and Methods Buffy coat–derived PCs were pooled and split to obtain matched pairs. One unit was treated using the Mirasol PRT system, while the control PC remained untreated. After 5 days of storage, either the PLTs or the PLT supernatants from the PCs were cocultured with PBMNCs , with or without lipopolysaccharide ( LPS ). The immunomodulatory factors secreted into culture medium after coculture were examined. Results PRT ‐treated PLTs and PLT supernatant significantly increased the interleukin ( IL )‐8 concentration, which was manifested only in the presence of LPS . Conversely, PRT ‐treated PLTs secreted less soluble P ‐selectin ( sCD 62 P ) upon coculture with PBMNCs . Conclusion PRT ‐treatment induced differential secretion of IL ‐8 and sCD 62 P during coculture, which may be attributed to either bioactive substances present in PLT supernatant or as a result of cell–cell interactions.